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Cold-shock proteome of myoblasts reveals role of RBM3 in promotion of mitochondrial metabolism and myoblast differentiation
Published in Communications Biology, 2024
This is joint work with Paulami Dey, Pushpita Saha, Purvi Singh Thakur, Maroof Athar Hashmi, Heera Lal, Nistha Saini, Nirpendra Singh and Arvind Ramanathan.
Recommended citation: Dey, P., Rajalaxmi, S., Saha, P. et al. Cold-shock proteome of myoblasts reveals role of RBM3 in promotion of mitochondrial metabolism and myoblast differentiation. Commun Biol 7, 515 (2024). https://doi.org/10.1038/s42003-024-06196-4
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Conserved and Unique Mitochondrial Target Sequence of TRPV4 Can Independently Regulate Mitochondrial Functions
Published in Proteins: Structure, Function, and Bioinformatics, 2024
Though mitochondria have their own genome and protein synthesis machineries, the majority of the mitochondrial proteins are actually encoded by the nuclear genome. Most of these mitochondrial proteins are imported into specific compartments of the mitochondria due to their mitochondrial target sequence (MTS). Unlike the nuclear target sequence, the MTS of most of the mitochondrial localized proteins remain poorly understood, mainly due to their variability, heterogeneity, unconventional modes of action, mitochondrial potential-dependent transport, and other complexities. Recently, we reported that transient receptor potential vanilloid subtype 4 (TRPV4), a thermosensitive cation channel, is physically located at the mitochondria. Here we char- acterize a small segment (AA 592- 630) located at the TM4-loop4-TM5 segment of TRPV4 that acts as a novel MTS. The same re- gion remains highly conserved in all vertebrates and contains a large number of point mutations each of which causes an diverse spectrum of diseases in human. Using confocal and super-resolution microscopy, we show that this MTS of TRPV4 or its mutants localizes to the mitochondria independently and also induces functional and quantitative changes in the mitochondria. By using conformal microscopy, we could detect the presence of the MTS region within the isolated mitochondria. These findings may be important to understand the complexity of MTS and TRPV4-induced channelopathies better.
Recommended citation: Acharya, T.K., Mahapatra, P., Kumar, S., Dubey, N.K., Rajalaxmi, S., Ghosh, A., Kumar, A. and Goswami, C. (2024), Conserved and Unique Mitochondrial Target Sequence of TRPV4 Can Independently Regulate Mitochondrial Functions. Proteins. https://doi.org/10.1002/prot.26772
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